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FACS analysis of pig peripheral blood using GTX29089 CD47 antibody [MEM-122]. Cells in the granulocyte gate were used for analysis. Panel A : pig PBMC stained with Isotype mouse IgM control Panel B : pig PBMC sample stained with GTX29089
FACS analysis of pig peripheral blood using GTX29089 CD47 antibody [MEM-122]. Cells in the granulocyte gate were used for analysis. Panel A : pig PBMC stained with Isotype mouse IgM control Panel B : pig PBMC sample stained with GTX29089
FACS analysis of pig peripheral blood using GTX29089 CD47 antibody [MEM-122]. Cells in the granulocyte gate were used for analysis. Panel A : pig PBMC stained with Isotype mouse IgM control Panel B : pig PBMC sample stained with GTX29089

CD47 antibody [MEM-122]

GTX29089
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetCD47
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD47 antibody [MEM-122]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MEM-122
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID961
  • Target name
    CD47
  • Target description
    CD47 molecule
  • Target synonyms
    IAP, MER6, OA3, leukocyte surface antigen CD47, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, Rh-related antigen, antigen identified by monoclonal antibody 1D8, antigenic surface determinant protein OA3, integrin associated protein, integrin-associated signal transducer
  • Host
    Mouse
  • Isotype
    IgM
  • Protein IDQ08722
  • Protein Name
    Leukocyte surface antigen CD47
  • Scientific Description
    This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD47 using anti-CD47 antibody (A00360-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD47 antigen affinity purified polyclonal antibody (Catalog # A00360-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD47 at approximately 41KD. The expected band size for CD47 is at 35KD.
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