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Cd68 Antibody

CSB-PA004951LA01MO
Cusabio
ApplicationsELISA
Product group Antibodies
TargetCd68
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Overview

  • Supplier
    Cusabio
  • Product Name
    Cd68 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID12514
  • Target name
    Cd68
  • Target description
    CD68 antigen
  • Target synonyms
    gp11; gp110; La; Lamp4; ma; macrosialin; Sca; Scard1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31996
  • Protein Name
    Macrosialin
  • Scientific Description
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (PA1518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (Catalog # PA1518) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.
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