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Formalin-fixed and paraffin embedded rat myocardium labeled with Anti-MuRF1/Trim63 Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat myocardium labeled with Anti-MuRF1/Trim63 Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat myocardium labeled with Anti-MuRF1/Trim63 Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

CD68 Polyclonal Antibody

BS-0649R
Bioss Antibodies
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCd68
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CD68 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID12514
  • Target name
    Cd68
  • Target description
    CD68 antigen
  • Target synonyms
    gp11; gp110; La; Lamp4; ma; macrosialin; Sca; Scard1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31996
  • Protein Name
    Macrosialin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Receptor activator of the NFkappaB ligand system protects renal function during experimental renal ischemia-reperfusion in mice. Nakayama Y et al., 2020 Feb, Transpl Immunol
    Read more
  • Isoflavone Ameliorated Oxidative Stress and Vascular Damages in Nicotine-Administrated Mice. Sukketsiri W et al., 2019 Dec 1, J Oleo Sci
    Read more
  • Fluoride Induces Autoimmune Orchitis Involved with Enhanced IL-17A Secretion in Mice Testis. Wu P et al., 2019 Dec 4, J Agric Food Chem
    Read more
  • Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively Activated Macrophages in Mice. Nishikawa S et al., 2019, J Nutr Sci Vitaminol (Tokyo)
    Read more
  • Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2-related factors in an ovalbumin-induced asthma mouse model. Bok SH et al., 2019 Oct, Mol Med Rep
    Read more
  • MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats. Luo Y et al., 2019 Mar, Brain Res Bull
    Read more
  • Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: A role in Kupffer cell activation. Zhang J et al., 2019 Mar 1, Toxicology
    Read more
  • Protective effects of dioscin against systemic inflammatory response syndromevia adjusting TLR2/MyD88/NF-kappab signal pathway. Zhao X et al., 2018 Dec, Int Immunopharmacol
    Read more
  • Radical polymerization-crosslinking method for improving extracellular matrix stability in bioprosthetic heart valves with reduced potential for calcification and inflammatory response. Guo G et al., 2018 Dec, Acta Biomater
    Read more
  • Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect. Zhou W et al., 2018 May 30, Biosens Bioelectron
    Read more

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Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (PA1518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (Catalog # PA1518) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.
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