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ProductsBack/CD70 antibody [FR70] (PerCP-Cy5.5)
FACS analysis of mouse C57Bl/6 splenocytes (LPS stimulated) using GTX01514-11 CD70 antibody [FR70] (PerCP-Cy5.5). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.5 microg (5 microl)
FACS analysis of mouse C57Bl/6 splenocytes (LPS stimulated) using GTX01514-11 CD70 antibody [FR70] (PerCP-Cy5.5). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.5 microg (5 microl)
FACS analysis of mouse C57Bl/6 splenocytes (LPS stimulated) using GTX01514-11 CD70 antibody [FR70] (PerCP-Cy5.5). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.5 microg (5 microl)

CD70 antibody [FR70] (PerCP-Cy5.5)

GTX01514-11
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd70
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD70 antibody [FR70] (PerCP-Cy5.5)
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FR70
  • Concentration
    0.2 mg/ml
  • Conjugate
    Double Conjugated
  • Gene ID21948
  • Target name
    Cd70
  • Target description
    CD70 antigen
  • Target synonyms
    CD27LG, Cd27l, Tnfsf7, Tnlg8a, CD70 antigen, CD27 ligand, CD27-L, Ki-24 antigen, tumor necrosis factor ligand 8a, tumor necrosis factor ligand superfamily member 7
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDO55237
  • Protein Name
    CD70 antigen
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Hanke NT, LaCasse CJ, Larmonier CB, et al. PIAS1 and STAT-3 impair the tumoricidal potential of IFN-γ-stimulated mouse dendritic cells generated with IL-15. Eur J Immunol. 2014,44(8):2489-2499. doi: 10.1002/eji.201343803
    Read this paper

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Figure 1. Western blot analysis of Cd70 using anti-Cd70 antibody (A02853-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse B16 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd70 antigen affinity purified polyclonal antibody (Catalog # A02853-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd70 at approximately 30 kDa. The expected band size for Cd70 is at 30 kDa.
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