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ProductsBack/ST6GAL1 antibody [N2C3]

ST6GAL1 antibody [N2C3]

GTX104018
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetST6GAL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD75 antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.44 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6480
  • Target name
    ST6GAL1
  • Target description
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1
  • Target synonyms
    alpha 2,6-ST 1; B-cell antigen CD75; beta-galactoside alpha-2,6-sialyltransferase 1; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); SIAT1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1; ST6GalI; ST6N
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15907
  • Protein Name
    Beta-galactoside alpha-2,6-sialyltransferase 1
  • Scientific Description
    This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • A set of cancer stem cell homing peptides associating with the glycan moieties of glycosphingolipids. Su YH et al., 2018 Apr 17, Oncotarget
    Read more

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Figure 1. Western blot analysis of CDw75/ST6GAL1 using anti-CDw75/ST6GAL1 antibody (A03156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDw75/ST6GAL1 antigen affinity purified polyclonal antibody (Catalog # A03156-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDw75/ST6GAL1 at approximately 47 kDa. The expected band size for CDw75/ST6GAL1 is at 47 kDa.
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