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Western Blot analysis of COLO cells using CD75 Polyclonal Antibody
Western Blot analysis of COLO cells using CD75 Polyclonal Antibody
Western Blot analysis of COLO cells using CD75 Polyclonal Antibody

ST6GAL1 Antibody

CSB-PA050064
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetST6GAL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ST6GAL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6480
  • Target name
    ST6GAL1
  • Target description
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1
  • Target synonyms
    alpha 2,6-ST 1; B-cell antigen CD75; beta-galactoside alpha-2,6-sialyltransferase 1; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); SIAT1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1; ST6GalI; ST6N
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15907
  • Protein Name
    Beta-galactoside alpha-2,6-sialyltransferase 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDw75/ST6GAL1 using anti-CDw75/ST6GAL1 antibody (A03156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDw75/ST6GAL1 antigen affinity purified polyclonal antibody (Catalog # A03156-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDw75/ST6GAL1 at approximately 47 kDa. The expected band size for CDw75/ST6GAL1 is at 47 kDa.
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