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ProductsBack/CD8 antibody [MEM-31] (PerCP)

CD8 antibody [MEM-31] (PerCP)

GTX79947
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetCD8A
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD8 antibody [MEM-31] (PerCP)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 10 microl reagent / 100 microl of whole blood or 106 cells in a suspension. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MEM-31
  • Conjugate
    PerCP
  • Gene ID925
  • Target name
    CD8A
  • Target description
    CD8a molecule
  • Target synonyms
    CD8; CD8 antigen, alpha polypeptide (p32); Leu2; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP01732
  • Protein Name
    T-cell surface glycoprotein CD8 alpha chain
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CD 8 alpha using anti-CD 8 alpha antibody (PB9249). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: JURKAT Whole Cell Lysate, Lane 2: RAJI Whole Cell Lysate, Lane 3: HL-60 Whole Cell Lysate, Lane 4: A549 Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD 8 alpha antigen affinity purified polyclonal antibody (Catalog # PB9249) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD 8 alpha at approximately 55 kDa. The expected band size for CD 8 alpha is at 26 kDa.
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