More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CD8 Antibody

CD8 Antibody

HY-P81148
MedChem Express
TargetCd8a
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    MedChem Express
  • Product Name
    CD8
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID12525
  • Target name
    Cd8a
  • Target description
    CD8 antigen, alpha chain
  • Target synonyms
    BB154331; Ly-; Ly-2; Ly-3; Ly-35; Ly-B; Lyt-; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CD8 Antibody is an unconjugated, approximately 27 kDa, rabbit-derived, anti-CD8 polyclonal antibody. CD8 Antibody can be used for: WB, IHC-P, IHC-F, IF expriments in mouse, background without labeling.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
FITC-Linked Anti-Cluster Of Differentiation 8a (CD8a) Polyclonal AntibodyCAU35251
ApplicationsFlow Cytometry
TargetCd8a
  • SizePrice
Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Product group Antibodies
References
Bioss Antibodies
CD8 Polyclonal AntibodyBS-0648R
ApplicationsFlow Cytometry
TargetCd8a
  • SizePrice
Product group Antibodies
GeneTex
CD8 alpha antibody [MAB0811]GTX52399
ApplicationsNeutralisation/Blocking, Other Application
TargetCd8a
  • SizePrice
4-20% SDS-PAGE Coomassie Staining
Product group Antibodies
BPS Bioscience
Anti-CD8 Antibody, Biotin-Labeled101779
TargetCd8a
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody [BLR173J]A700-173
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetCd8a
  • SizePrice
Product group Antibodies
Biosynth
CD8a antibody10R-CD8ABMS
  • SizePrice
Binding of anti-mCD8alpha (53-6.72) +GAR/FITC to balb-c splenocytes. Method: Tested for binding to ACK lysed murine splenocytes in FACS. Five x 10^5 splenocytes per tube were washed and pre-incubated with 20ul of 300ug/ml murine IgG (to reduce non-specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD8 antibody diluted to 10ug/ml. Cells were washed twice and incubated with 2nd reagent Goat anti-Rat IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. An 8.8 % sub population of the cells stained positive with a mean shift of 1.6 log10 fluorescent units when compared to background.
Product group Antibodies
Ancell Corporation
anti-CD8-alpha (mouse), mAb (53-6) (preservative free)ANC-260-820
ApplicationsFlow Cytometry, ELISA
TargetCd8a
  • SizePrice
Product group Antibodies
Assay Genie
Purified Anti-Mouse CD8a Antibody [53-6.7]AGEL0378
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
TargetCd8a
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CD8 AntibodyLS-C485260
ApplicationsFlow Cytometry
  • SizePrice
Figure 1. Western blot analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate, Lane 2: rat thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8 alpha antigen affinity purified polyclonal antibody (Catalog # A02236-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8 alpha at approximately 36KD. The expected band size for CD8 alpha is at 26KD.
Product group Antibodies
Boster Bio
Anti-CD8 alpha/Cd8a Antibody Picoband(r)A02236-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetCd8a
  • SizePrice