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Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

CD8 Polyclonal Antibody

BS-0648R
Bioss Antibodies
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd8a
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CD8 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow-Cyt(1:50)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID12525
  • Target name
    Cd8a
  • Target description
    CD8 antigen, alpha chain
  • Target synonyms
    BB154331; Ly-; Ly-2; Ly-3; Ly-35; Ly-B; Lyt-; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01731
  • Protein Name
    T-cell surface glycoprotein CD8 alpha chain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Knockout of beta-2 microglobulin reduces stem cell-induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR-24/Bim pathway. Zhang Y et al., 2020 Jan, J Cell Mol Med
    Read more
  • Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer. Yuan SJ et al., 2019 Oct 17, J Nanobiotechnology
    Read more
  • Hypotonic stress enhances colon cancer cell death induced by platinum derivatives and immunologically improves antitumor efficacy of intraperitoneal chemotherapy. Demontoux L et al., 2019 Dec 1, Int J Cancer
    Read more
  • Doxorubicin and siRNA-PD-L1 co-delivery with T7 modified ROS-sensitive nanoparticles for tumor chemoimmunotherapy. Wan WJ et al., 2019 Jul 20, Int J Pharm
    Read more
  • Clinical effects of lentinan combined with budesonide inhalation in treating acute exacerbation of chronic obstructive pulmonary disease under mechanical ventilation. Sun J et al., 2019 Mar, Exp Ther Med
    Read more
  • Utilizing VEGF165b mutant as an effective immunization adjunct to augment antitumor immune response. Zhang H et al., 2019 Apr 3, Vaccine
    Read more
  • Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture Healing. Moedinger Y et al., 2019 Jan, Am J Pathol
    Read more
  • Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma. Li TF et al., 2018 Oct, Biomaterials
    Read more
  • Black tea and D. candidum extracts play estrogenic activity via estrogen receptor alpha-dependent signaling pathway. Wang Y et al., 2018, Am J Transl Res
    Read more
  • Co-delivery of tumor antigen and dual toll-like receptor ligands into dendritic cell by silicon microparticle enables efficient immunotherapy against melanoma. Zhu M et al., 2018 Feb 28, J Control Release
    Read more

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Figure 1. Western blot analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate, Lane 2: rat thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8 alpha antigen affinity purified polyclonal antibody (Catalog # A02236-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8 alpha at approximately 36KD. The expected band size for CD8 alpha is at 26KD.
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