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ProductsBack/Cd86 Rat Monoclonal Antibody [Clone ID: GL1]
Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Rat anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 ug/10e6 cells.
Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Rat anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: &lt; / = 1 ug/10e6 cells.
Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Rat anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 ug/10e6 cells.

Cd86 Rat Monoclonal Antibody [Clone ID: GL1]

AM08053FC-N
OriGene
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetCd86
Price on request
500 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Cd86 Rat Monoclonal Antibody [Clone ID: GL1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GL1
  • Gene ID12524
  • Target name
    Cd86
  • Target description
    CD86 antigen
  • Target synonyms
    B7, B7-2, B7.2, B70, CLS1, Cd28l2, ETC-1, Ly-58, Ly58, MB7, MB7-2, TS/A-2, T-lymphocyte activation antigen CD86, activation B7-2 antigen, early T cell costimulatory molecule-1
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP42082
  • Protein Name
    T-lymphocyte activation antigen CD86
  • Scientific Description
    Cd86 rat monoclonal antibody, clone GL1, FITC
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Cd86 using anti-Cd86 antibody (A00220-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd86 antigen affinity purified polyclonal antibody (Catalog # A00220-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd86 at approximately 60-80 kDa. The expected band size for Cd86 is at 35 kDa.
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