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ProductsBack/CD89 (A3): m-IgGkappa BP-HRP Bundle

CD89 (A3): m-IgGkappa BP-HRP Bundle

SC-520697
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
TargetFCAR
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    CD89 (A3): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2204
  • Target name
    FCAR
  • Target description
    Fc alpha receptor
  • Target synonyms
    CD89, CTB-61M7.2, FcalphaR, FcalphaRI, immunoglobulin alpha Fc receptor, Fc fragment of IgA receptor, Fc fragment of IgA, receptor for
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD89/FCAR using anti-CD89/FCAR antibody (A07546-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD89/FCAR antigen affinity purified polyclonal antibody (Catalog # A07546-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD89/FCAR at approximately 32 kDa. The expected band size for CD89/FCAR is at 32 kDa.
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