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ProductsBack/CD95 / FAS Antibody

CD95 / FAS Antibody

LS-C812965
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetFAS
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD95 / FAS Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID355
  • Target name
    FAS
  • Target description
    Fas cell surface death receptor
  • Target synonyms
    ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, tumor necrosis factor receptor superfamily member 6, APO-1 cell surface antigen, CD95 antigen, FASLG receptor, Fas (TNF receptor superfamily, member 6), Fas AMA, TNF receptor superfamily member 6, apoptosis antigen 1, apoptosis signaling receptor FAS, apoptosis-mediating surface antigen FAS, mutant tumor necrosis receptor superfamily member 6, tumor necrosis factor receptor superfamily, member 6
  • Host
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Fas using anti-Fas antibody (A00055). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fas antigen affinity purified polyclonal antibody (Catalog # A00055) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fas at approximately 37KD. The expected band size for Fas is at 37KD.
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