Boiled and unboiled U937 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637674/GTX637674_T-44837_20230414_WB_B_23041723_947.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Immunoprecipitation of CD97 protein from U937 using 5 μg of CD97 antibody [HL1925] (GTX637674). Western blot analysis was performed using CD97 antibody [HL1925] (GTX637674). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637674/GTX637674_44900_20230616_IP_23061400_905.webp "Immunoprecipitation of CD97 protein from U937 using 5 μg of CD97 antibody [HL1925] (GTX637674). Western blot analysis was performed using CD97 antibody [HL1925] (GTX637674). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).")
![Untreated (–) and treated (+) U937 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637674/GTX637674_T-44837_20230626_WB_treatment_Tunicamycin_23062800_878.webp "Untreated (–) and treated (+) U937 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637674/GTX637674_44900_20240105_WB_TPM_watermark_24010821_298.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637674/GTX637674_45271_20240119_WB_KO_watermark_24031823_914.webp "Wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Wild-type (WT) and CD97 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and the highly cited competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637674/GTX637674_45271_20240301_WB_KO_competitor_watermark_24031823_899.webp "Wild-type (WT) and CD97 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and the highly cited competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![U937 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and competitor's antibody (Clone EPR4427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637674/GTX637674_45271_20240223_WB_Fraction_competitor_watermark_24031823_952.webp "U937 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:1000 and competitor's antibody (Clone EPR4427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.")
Boiled and unboiled U937 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD97 antibody [HL1925] (GTX637674) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
CD97 antibody [HL1925]
GTX637674
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetADGRE5
Overview
- SupplierGeneTex
- Product NameCD97 antibody [HL1925]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1925
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID976
- Target nameADGRE5
- Target descriptionadhesion G protein-coupled receptor E5
- Target synonymsCD97, TM7LN1, adhesion G protein-coupled receptor E5, CD97 molecule, leukocyte antigen CD97, seven transmembrane helix receptor, seven-span transmembrane protein, seven-transmembrane, heterodimeric receptor associated with inflammation
- HostRabbit
- IsotypeIgG
- Protein IDP48960
- Protein NameAdhesion G protein-coupled receptor E5
- Scientific DescriptionThis gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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