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CD97 Rabbit pAb

A3780
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetADGRE5
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Overview

  • Supplier
    ABclonal
  • Product Name
    CD97 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID976
  • Target name
    ADGRE5
  • Target description
    adhesion G protein-coupled receptor E5
  • Target synonyms
    CD97, TM7LN1, adhesion G protein-coupled receptor E5, CD97 molecule, leukocyte antigen CD97, seven transmembrane helix receptor, seven-span transmembrane protein, seven-transmembrane, heterodimeric receptor associated with inflammation
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48960
  • Protein Name
    Adhesion G protein-coupled receptor E5
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ADGRE5 using anti-ADGRE5 antibody (A30392-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADGRE5 antigen affinity purified polyclonal antibody (Catalog # A30392-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADGRE5 at approximately 98 kDa. The expected band size for ADGRE5 is at 92 kDa.
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Western blot All lanes: CD97 antibody at 3.11ug/ml Lane 1: HL60 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 92, 82, 87 kDa Observed band size: 92 kDa
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