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ProductsBack/CDC25B Antibody
Western Blot Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate All lanes: CDC25B antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 64, 61, 68 kDa Observed band size: 65 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate All lanes: CDC25B antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 64, 61, 68 kDa Observed band size: 65 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate All lanes: CDC25B antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 64, 61, 68 kDa Observed band size: 65 kDa

CDC25B Antibody

CSB-PA004995LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCDC25B
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDC25B Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID994
  • Target name
    CDC25B
  • Target description
    cell division cycle 25B
  • Target synonyms
    CDC25 homolog B; dual specificity phosphatase Cdc25B; M-phase inducer phosphatase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30305
  • Protein Name
    M-phase inducer phosphatase 2
  • Scientific Description
    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDC25B using anti-CDC25B antibody (A01899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human Hacat whole cell lysates. Lane 7: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25B antigen affinity purified polyclonal antibody (Catalog # A01899-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC25B at approximately 70 kDa. The expected band size for CDC25B is at 65 kDa.
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