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CDC25B Polyclonal Antibody

CAC14584
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCDC25B
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Overview

  • Supplier
    Biomatik
  • Product Name
    CDC25B Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID994
  • Target name
    CDC25B
  • Target description
    cell division cycle 25B
  • Target synonyms
    CDC25 homolog B; dual specificity phosphatase Cdc25B; M-phase inducer phosphatase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30305
  • Protein Name
    M-phase inducer phosphatase 2
  • Scientific Description
    The CDC25B Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDC25B using anti-CDC25B antibody (A01899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human Hacat whole cell lysates. Lane 7: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25B antigen affinity purified polyclonal antibody (Catalog # A01899-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC25B at approximately 70 kDa. The expected band size for CDC25B is at 65 kDa.
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