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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA169739(CDC25B Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA169739(CDC25B Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA169739(CDC25B Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)

CDC25B Antibody

CSB-PA169739
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCDC25B
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDC25B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID994
  • Target name
    CDC25B
  • Target description
    cell division cycle 25B
  • Target synonyms
    MPIP2, M-phase inducer phosphatase 2, CDC25 homolog B, dual specificity phosphatase Cdc25B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30305
  • Protein Name
    M-phase inducer phosphatase 2
  • Scientific Description
    CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDC25B using anti-CDC25B antibody (A01899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human Hacat whole cell lysates. Lane 7: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25B antigen affinity purified polyclonal antibody (Catalog # A01899-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC25B at approximately 70 kDa. The expected band size for CDC25B is at 65 kDa.
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