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Western Blot analysis of COLO205 cells using Cdc27 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Cdc27 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Cdc27 Polyclonal Antibody

CDC27 Antibody

CSB-PA001504
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDC27
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDC27 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID996
  • Target name
    CDC27
  • Target description
    cell division cycle 27
  • Target synonyms
    ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2, cell division cycle protein 27 homolog, D0S1430E, D17S978E, anaphase promoting complex subunit 3, anaphase-promoting complex, protein 3, cell division cycle 27 homolog, nuc2 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30260
  • Protein Name
    Cell division cycle protein 27 homolog
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDC27 using anti-CDC27 antibody (A03905-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC27 antigen affinity purified polyclonal antibody (Catalog # A03905-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC27 at approximately 92KD. The expected band size for CDC27 is at 92KD.
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