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CDC2L5 antibody

GTX102408
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCDK13
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Overview

  • Supplier
    GeneTex
  • Product Name
    CDC2L5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.83 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8621
  • Target name
    CDK13
  • Target description
    cyclin dependent kinase 13
  • Target synonyms
    CDC2L; CDC2L5; CDC2-related protein kinase 5; cell division cycle 2-like protein kinase 5; cell division protein kinase 13; CHDFIDD; CHED; cholinesterase-related cell division controller; cyclin-dependent kinase 13; hCDK13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14004
  • Protein Name
    Cyclin-dependent kinase 13
  • Scientific Description
    The protein encoded by this gene is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are well known for their essential roles as master switches in cell cycle control. Some of the cell cycle control kinases are able to phosphorylate proteins that are important for cell differentiation and apoptosis, thus provide connections between cell proliferation, differentiation, and apoptosis. Proteins of this family may also be involved in non-cell cycle-related functions, such as neurocytoskeleton dynamics. The exact function of this protein has not yet been determined. It has unusually large N- and C-termini and is ubiquitously expressed in many tissues. Two alternatively spliced variants are described. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Topography of transcriptionally active chromatin in glioblastoma. Xu L et al., 2021 Apr, Sci Adv
    Read more
  • CDK12 drives breast tumor initiation and trastuzumab resistance via WNT and IRS1-ErbB-PI3K signaling. Choi HJ et al., 2019 Oct 4, EMBO Rep
    Read more

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Figure 1. Western blot analysis of CDK13 using anti-CDK13 antibody (A05292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK13 antigen affinity purified polyclonal antibody (Catalog # A05292-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK13 at approximately 185-190KD. The expected band size for CDK13 is at 165KD.
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