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Western blot All lanes: CDC45 antibody at 1.05microg/ml Lane 1: K562 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 66, 61, 69 kDa Observed band size: 66, 69 kDa
Western blot All lanes: CDC45 antibody at 1.05microg/ml Lane 1: K562 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 66, 61, 69 kDa Observed band size: 66, 69 kDa
Western blot All lanes: CDC45 antibody at 1.05microg/ml Lane 1: K562 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 66, 61, 69 kDa Observed band size: 66, 69 kDa

CDC45 Antibody

CSB-PA005020ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCDC45
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDC45 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8318
  • Target name
    CDC45
  • Target description
    cell division cycle 45
  • Target synonyms
    CDC45 cell division cycle 45 homolog; CDC45 cell division cycle 45-like; CDC45L; CDC45L2; CDC45-related protein; cell division control protein 45 homolog; cell division cycle 45 homolog; cell division cycle 45-like 2; human CDC45; MGORS7; PORC-PI-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75419
  • Protein Name
    Cell division control protein 45 homolog
  • Scientific Description
    Required for initiation of chromosomal DNA replication.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDC45L using anti-CDC45L antibody (A01367-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC45L antigen affinity purified polyclonal antibody (Catalog # A01367-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC45L at approximately 66KD. The expected band size for CDC45L is at 66KD.
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