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CDC45 Antibody

LS-C331850
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCDC45
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDC45 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 60kDa/65kDa/68kDa, while the observed MW by Western blot was 60kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 60kDa/65kDa/68kDa, while the observed MW by Western blot was 60kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8318
  • Target name
    CDC45
  • Target description
    cell division cycle 45
  • Target synonyms
    CDC45L, CDC45L2, MGORS7, PORC-PI-1, cell division control protein 45 homolog, CDC45 cell division cycle 45 homolog, CDC45 cell division cycle 45-like, CDC45-related protein, cell division cycle 45 homolog, cell division cycle 45-like 2, human CDC45
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDC45L using anti-CDC45L antibody (A01367-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC45L antigen affinity purified polyclonal antibody (Catalog # A01367-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC45L at approximately 66KD. The expected band size for CDC45L is at 66KD.
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