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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA039310(CDC7 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA039310(CDC7 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA039310(CDC7 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)

CDC7 Antibody

CSB-PA039310
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCDC7
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDC7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8317
  • Target name
    CDC7
  • Target description
    cell division cycle 7
  • Target synonyms
    CDC7L1, HsCDC7, Hsk1, huCDC7, cell division cycle 7-related protein kinase, CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1, CDC7-related kinase, cell division cycle 7 homolog, cell division cycle 7-like protein 1, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00311
  • Protein Name
    Cell division cycle 7-related protein kinase
  • Scientific Description
    This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDC7 using anti-CDC7 antibody (A01190-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC7 antigen affinity purified polyclonal antibody (Catalog # A01190-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC7 at approximately 64 kDa. The expected band size for CDC7 is at 64 kDa.
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