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CDCA5 / Sororin Antibody

LS-C664515
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCDCA5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDCA5 / Sororin Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID113130
  • Target name
    CDCA5
  • Target description
    cell division cycle associated 5
  • Target synonyms
    SORORIN, sororin, cell division cycle-associated protein 5, p35
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDCA5 using anti-CDCA5 antibody (M05043). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCA5 antigen affinity purified monoclonal antibody (Catalog # M05043) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCA5 at approximately 35 kDa. The expected band size for CDCA5 is at 28 kDa.
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