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Western Blot analysis of HeLa cells using CDCP1 Polyclonal Antibody
Western Blot analysis of HeLa cells using CDCP1 Polyclonal Antibody
Western Blot analysis of HeLa cells using CDCP1 Polyclonal Antibody

CDCP1 Antibody

CSB-PA001521
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetCDCP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDCP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64866
  • Target name
    CDCP1
  • Target description
    CUB domain containing protein 1
  • Target synonyms
    CD318, SIMA135, TRASK, CUB domain-containing protein 1, membrane glycoprotein gp140, subtractive immunization M plus HEp3-associated 135 kDa protein, transmembrane and associated with src kinases
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H5V8
  • Protein Name
    CUB domain-containing protein 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDCP1 using anti-CDCP1 antibody (A02411-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCP1 antigen affinity purified polyclonal antibody (Catalog # A02411-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCP1 at approximately 130 kDa. The expected band size for CDCP1 is at 93 kDa.
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