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Western Blot analysis of MCF7 K562 cells using Cdk4 Polyclonal Antibody
Western Blot analysis of MCF7 K562 cells using Cdk4 Polyclonal Antibody
Western Blot analysis of MCF7 K562 cells using Cdk4 Polyclonal Antibody

CDK4 Antibody

CSB-PA005118
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDK4
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDK4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1019
  • Target name
    CDK4
  • Target description
    cyclin dependent kinase 4
  • Target synonyms
    cell division protein kinase 4; CMM3; cyclin-dependent kinase 4; PSK-J3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11802
  • Protein Name
    Cyclin-dependent kinase 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDK4 using anti-CDK4 antibody (PA1428-1, Left) and anti-CDK4 antibody (PB9535, Right). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mosue NIH/3T3 whole cell lysates, Lane 5: mosue RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PA1428-1) and rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PB9535)at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)?with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.
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