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ProductsBack/CDK4 + CDK6 antibody [HL2323]

CDK4 + CDK6 antibody [HL2323]

GTX638486
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDK4
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Overview

  • Supplier
    GeneTex
  • Product Name
    CDK4 + CDK6 antibody [HL2323]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2323
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1019
  • Target name
    CDK4
  • Target description
    cyclin dependent kinase 4
  • Target synonyms
    CMM3, PSK-J3, cyclin-dependent kinase 4, cell division protein kinase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11802
  • Protein Name
    Cyclin-dependent kinase 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CDK4 using anti-CDK4 antibody (PA1428-1, Left) and anti-CDK4 antibody (PB9535, Right). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mosue NIH/3T3 whole cell lysates, Lane 5: mosue RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PA1428-1) and rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PB9535)at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)?with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.
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