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ProductsBack/CDK5 Monoclonal Antibody
Lane 1: SH-SY5Y cell lysates; Lane 2: SK-BR-3 cell lysatess; Lane 3: Hela cell lysatess; Lane 4: Mouse brain tissue lysatess; Lane 5: Rat brain tissue lysatess probed with CDK5 Monoclonal Antibody, Unconjugated (bsm-51583M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: SH-SY5Y cell lysates; Lane 2: SK-BR-3 cell lysatess; Lane 3: Hela cell lysatess; Lane 4: Mouse brain tissue lysatess; Lane 5: Rat brain tissue lysatess probed with CDK5 Monoclonal Antibody, Unconjugated (bsm-51583M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: SH-SY5Y cell lysates; Lane 2: SK-BR-3 cell lysatess; Lane 3: Hela cell lysatess; Lane 4: Mouse brain tissue lysatess; Lane 5: Rat brain tissue lysatess probed with CDK5 Monoclonal Antibody, Unconjugated (bsm-51583M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

CDK5 Monoclonal Antibody

BSM-51583M
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetCDK5
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Overview

  • Supplier
    Bioss
  • Product Name
    CDK5 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-1000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:400-800), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    H11F1
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID1020
  • Target name
    CDK5
  • Target description
    cyclin dependent kinase 5
  • Target synonyms
    LIS7, PSSALRE, cyclin-dependent kinase 5, TPKII catalytic subunit, cell division protein kinase 5, cyclin-dependent-like kinase 5, epididymis secretory sperm binding protein, protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE, tau protein kinase II catalytic subunit
  • Host
    Mouse
  • Protein IDQ00535
  • Protein Name
    Cyclin-dependent kinase 5
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CDK5 using anti-CDK5 antibody (A00511-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK5 antigen affinity purified polyclonal antibody (Catalog # A00511-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK5 at approximately 33 kDa. The expected band size for CDK5 is at 33 kDa.
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