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ProductsBack/CDK5 Mouse Monoclonal Antibody [Clone ID: 4E4]
Western blot analysis using CDK5 mouse mAb against Hela (1), K562 (2), PC-12 (3) and Cos7 (4) cell lysate.
Western blot analysis using CDK5 mouse mAb against Hela (1), K562 (2), PC-12 (3) and Cos7 (4) cell lysate.
Western blot analysis using CDK5 mouse mAb against Hela (1), K562 (2), PC-12 (3) and Cos7 (4) cell lysate.

CDK5 Mouse Monoclonal Antibody [Clone ID: 4E4]

AM06622SU-N
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Rat
TargetCDK5
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    CDK5 Mouse Monoclonal Antibody [Clone ID: 4E4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4E4
  • Gene ID1020
  • Target name
    CDK5
  • Target description
    cyclin dependent kinase 5
  • Target synonyms
    LIS7, PSSALRE, cyclin-dependent kinase 5, TPKII catalytic subunit, cell division protein kinase 5, cyclin-dependent-like kinase 5, epididymis secretory sperm binding protein, protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE, tau protein kinase II catalytic subunit
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ00535
  • Protein Name
    Cyclin-dependent kinase 5
  • Scientific Description
    CDK5 mouse monoclonal antibody, clone 4E4, Ascites
  • Reactivity
    Human, Monkey, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDK5 using anti-CDK5 antibody (A00511-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK5 antigen affinity purified polyclonal antibody (Catalog # A00511-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK5 at approximately 33 kDa. The expected band size for CDK5 is at 33 kDa.
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