Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK7 antibody (GTX111919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![CDK7 antibody immunoprecipitates CDK7 protein in IP experiments. IP samples: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of CDK7 protein by 4 μg CDK7 antibody (GTX111919) 10 % SDS-PAGE The immunoprecipitated CDK7 protein was detected by CDK7 antibody (GTX111919) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX111919/GTX111919_40073_IP_w_23060500_367.webp "CDK7 antibody immunoprecipitates CDK7 protein in IP experiments. IP samples: 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of CDK7 protein by 4 μg CDK7 antibody (GTX111919) 10 % SDS-PAGE The immunoprecipitated CDK7 protein was detected by CDK7 antibody (GTX111919) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK7 antibody (GTX111919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CDK7 antibody
GTX111919
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCDK7
Overview
- SupplierGeneTex
- Product NameCDK7 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.26 mg/ml
- ConjugateUnconjugated
- Gene ID1022
- Target nameCDK7
- Target descriptioncyclin dependent kinase 7
- Target synonymsCAK, CAK1, CDKN7, HCAK, MO15, STK1, p39MO15, cyclin-dependent kinase 7, 39 KDa protein kinase, CDK-activating kinase 1, TFIIH basal transcription factor complex kinase subunit, cell division protein kinase 7, cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase), homolog of Xenopus MO15 Cdk-activating kinase, kinase subunit of CAK, serine/threonine kinase stk1, serine/threonine protein kinase 1, serine/threonine protein kinase MO15
- HostRabbit
- IsotypeIgG
- Protein IDP50613
- Protein NameCyclin-dependent kinase 7
- Scientific DescriptionThe protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8Read this paper
- Narita T, Narita K, Takedachi A, et al. Regulation of Transcription Elongation by the XPG-TFIIH Complex Is Implicated in Cockayne Syndrome. Mol Cell Biol. 2015,35(18):3178-88. doi: 10.1128/MCB.01401-14Read this paper
Datasheet
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