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Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDK7 Polyclonal Antibody, Unconjugated (bs-0569R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDK7 Polyclonal Antibody, Unconjugated (bs-0569R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDK7 Polyclonal Antibody, Unconjugated (bs-0569R) at 1:200 overnight at 4°C, DAB staining.

CDK7 Polyclonal Antibody

BS-0569R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCDK7
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CDK7 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1022
  • Target name
    CDK7
  • Target description
    cyclin dependent kinase 7
  • Target synonyms
    39 KDa protein kinase; CAK; CAK1; CDK-activating kinase 1; CDKN7; cell division protein kinase 7; cyclin-dependent kinase 7; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); HCAK; homolog of Xenopus MO15 Cdk-activating kinase; kinase subunit of CAK; MO15; p39MO15; serine/threonine kinase stk1; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; STK1; TFIIH basal transcription factor complex kinase subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50613
  • Protein Name
    Cyclin-dependent kinase 7
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Cdk7 using anti-Cdk7 antibody (PB9536). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug, Lane 4: HEPG2 Whole Cell Lysate at 40ug, Lane 5: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk7 antigen affinity purified polyclonal antibody (Catalog # PB9536) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk7 at approximately 39 kDa. The expected band size for Cdk7 is at 39 kDa.
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