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CDK8 Rabbit mAb

A9654
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDK8
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Overview

  • Supplier
    ABclonal
  • Product Name
    CDK8 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID1024
  • Target name
    CDK8
  • Target description
    cyclin dependent kinase 8
  • Target synonyms
    IDDHBA, K35, cyclin-dependent kinase 8, CDK8 protein kinase, cell division protein kinase 8, mediator complex subunit CDK8, mediator of RNA polymerase II transcription subunit CDK8, protein kinase K35
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49336
  • Protein Name
    Cyclin-dependent kinase 8
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDK8 using anti-CDK8 antibody (A01493-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK8 antigen affinity purified polyclonal antibody (Catalog # A01493-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK8 at approximately 53 kDa. The expected band size for CDK8 is at 53 kDa.
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