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WB analysis of human ovary lysate using GTX14676 CDT1 antibody, C-term. Dilution : 0.05microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human ovary lysate using GTX14676 CDT1 antibody, C-term. Dilution : 0.05microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human ovary lysate using GTX14676 CDT1 antibody, C-term. Dilution : 0.05microg/ml Loading : 35microg protein in RIPA buffer

CDT1 antibody, C-term

GTX14676
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCDT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CDT1 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID81620
  • Target name
    CDT1
  • Target description
    chromatin licensing and DNA replication factor 1
  • Target synonyms
    DNA replication factor Cdt1; Double parked, Drosophila, homolog of; DUP; RIS2
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9H211
  • Protein Name
    DNA replication factor Cdt1
  • Scientific Description
    The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein. [provided by RefSeq, Mar 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDT1/DUP using anti-CDT1/DUP antibody (A01035-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDT1/DUP antigen affinity purified polyclonal antibody (Catalog # A01035-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDT1/DUP at approximately 60 kDa. The expected band size for CDT1/DUP is at 60 kDa.
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