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Western Blot analysis of HepG2 cells using CEACAM1/5 Polyclonal Antibody
Western Blot analysis of HepG2 cells using CEACAM1/5 Polyclonal Antibody
Western Blot analysis of HepG2 cells using CEACAM1/5 Polyclonal Antibody

CEACAM1 Antibody

CSB-PA005134
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCEACAM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CEACAM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID634
  • Target name
    CEACAM1
  • Target description
    CEA cell adhesion molecule 1
  • Target synonyms
    antigen CD66; BGP; BGP1; BGPI; carcinoembryonic antigen related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); CD66a antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13688
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG22 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 120-150 kDa. The expected band size for CEACAM1 is at 58 kDa.
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