C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (GTX100674) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (GTX100674) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100674/GTX100674_40058_20160331_WB_M_w_23060100_816.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100674/GTX100674_43586_20230630_WB_H_M_TPM_watermark_23062718_147.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (GTX100674) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (GTX100674) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
C/EBP alpha antibody [N1], N-term
GTX100674
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Sheep
TargetCEBPA
Overview
- SupplierGeneTex
- Product NameC/EBP alpha antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.59 mg/ml
- ConjugateUnconjugated
- Gene ID1050
- Target nameCEBPA
- Target descriptionCCAAT enhancer binding protein alpha
- Target synonymsC/EBP-alpha, CEBP, CCAAT/enhancer-binding protein alpha, CCAAT/enhancer binding protein (C/EBP), alpha
- HostRabbit
- IsotypeIgG
- Protein IDP49715
- Protein NameCCAAT/enhancer-binding protein alpha
- Scientific DescriptionThe protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq]
- ReactivityHuman, Mouse, Sheep
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Yang H, Ma C, Zi Y, et al. Effects of maternal undernutrition during late pregnancy on the regulatory factors involved in growth and development in ovine fetal perirenal brown adipose tissue. Anim Biosci. 2022,35(7):1010-1020. doi: 10.5713/ab.21.0199Read this paper
- Liu H, Zhan YL, Luo GJ, et al. Liraglutide and Insulin Have Contrary Effects on Adipogenesis of Human Adipose-Derived Stem Cells via Wnt Pathway. Diabetes Metab Syndr Obes. 2020,13:3075-3087. doi: 10.2147/DMSO.S253097Read this paper
- Chen CC, Lee TY, Leu YL, et al. Pigment epithelium-derived factor inhibits adipogenesis in 3T3-L1 adipocytes and protects against high-fat diet-induced obesity and metabolic disorders in mice. Transl Res. 2019,210:26-42. doi: 10.1016/j.trsl.2019.04.006Read this paper
- de Kerckhove M, Tanaka K, Umehara T, et al. Targeting miR-223 in neutrophils enhances the clearance of Staphylococcus aureus in infected wounds. EMBO Mol Med. 2018,10(10). doi: 10.15252/emmm.201809024Read this paper
- Wu JS, Kao MH, Tsai HD, et al. Clinacanthus nutans Mitigates Neuronal Apoptosis and Ischemic Brain Damage Through Augmenting the C/EBPβ-Driven PPAR-γ Transcription. Mol Neurobiol. 2018,55(7):5425-5438. doi: 10.1007/s12035-017-0776-zRead this paper
- Chen WC, Tseng CK, Chen YH, et al. HCV NS5A Up-Regulates COX-2 Expression via IL-8-Mediated Activation of the ERK/JNK MAPK Pathway. PLoS One. 2015,10(7):e0133264. doi: 10.1371/journal.pone.0133264Read this paper
- Chen YF, Chong CL, Wu YC, et al. Doxorubicin Activates Hepatitis B Virus Replication by Elevation of p21 (Waf1/Cip1) and C/EBPα Expression. PLoS One. 2015,10(6):e0131743. doi: 10.1371/journal.pone.0131743Read this paper
Datasheet
Related products
Product group Antibodies
Anti-CEBPA Antibody144-61658
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCEBPA
- SizePrice
Product group Antibodies
Anti-CEBP Alpha/CEBPA Antibody Picoband(r)A00386-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetCEBPA
- SizePrice
![ELISA analysis of antigen using GTX60578 C/EBP alpha antibody [4E10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60578/GTX60578_20170912_ELISA_w_23061123_604.webp)
Product group Antibodies
C/EBP alpha antibody [4E10]GTX60578
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetCEBPA
- SizePrice
![ELISA analysis of antigen using GTX60584 C/EBP alpha antibody [5B7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60584/GTX60584_20170912_ELISA_w_23061123_647.webp)
Product group Antibodies
C/EBP alpha antibody [5B7]GTX60584
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCEBPA
- SizePrice
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (GTX637397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637397/GTX637397_44837_20221021_WB_TPM_watermark_22102723_332.webp)
Product group Antibodies
C/EBP alpha antibody [HL1747]GTX637397
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Rat
TargetCEBPA
- SizePrice
Product group Antibodies
CEBPA Polyclonal AntibodyCAC14713
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCEBPA
- SizePrice
Product group Antibodies
References
CEBP-alpha Polyclonal AntibodyBS-1630R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetCEBPA
- SizePrice
Product group Antibodies
Anti-CEBPA AntibodyA46053
ApplicationsImmunoHistoChemistry
ReactivityHuman
- SizePrice
Product group Antibodies
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCEBPA
- SizePrice