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Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-CEBP-alpha Polyclonal Antibody, Unconjugated (bs-1630R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-CEBP-alpha Polyclonal Antibody, Unconjugated (bs-1630R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-CEBP-alpha Polyclonal Antibody, Unconjugated (bs-1630R) followed by conjugation to the secondary antibody and DAB staining

CEBP-alpha Polyclonal Antibody

BS-1630R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetCEBPA
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Overview

  • Supplier
    Bioss
  • Product Name
    CEBP-alpha Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1050
  • Target name
    CEBPA
  • Target description
    CCAAT enhancer binding protein alpha
  • Target synonyms
    C/EBP-alpha, CEBP, CCAAT/enhancer-binding protein alpha, CCAAT/enhancer binding protein (C/EBP), alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49715
  • Protein Name
    CCAAT/enhancer-binding protein alpha
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhang K, Wang F, Huang J, et al. Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells. Exp Ther Med. 2019,17(3):1663-1669. doi: 10.3892/etm.2018.7132
    Read this paper
  • Wang F, Li H, Lou Y, et al. Insulin‑like growth factor I promotes adipogenesis in hemangioma stem cells from infantile hemangiomas. Mol Med Rep. 2019,19(4):2825-2830. doi: 10.3892/mmr.2019.9895
    Read this paper

Datasheet

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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-CEBP Alpha/CEBPA antibody (A00386-1). Overlay histogram showing CACO-2 cells stained with A00386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBP Alpha/CEBPA Antibody (A00386-1, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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