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ProductsBack/C/EBP Alpha / CEBPA Antibody (clone 5B7)

C/EBP Alpha / CEBPA Antibody (clone 5B7)

LS-C169188
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCEBPA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    C/EBP Alpha / CEBPA Antibody (clone 5B7)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), IHC, IHC-P (1:200 - 1:1000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B7
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1050
  • Target name
    CEBPA
  • Target description
    CCAAT enhancer binding protein alpha
  • Target synonyms
    C/EBP-alpha; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer-binding protein alpha; CEBP
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-CEBP Alpha/CEBPA antibody (A00386-1). Overlay histogram showing CACO-2 cells stained with A00386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBP Alpha/CEBPA Antibody (A00386-1, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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