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ProductsBack/C/EBP Beta / CEBPB Antibody (clone 1D5-E5-G8, Preservative Free)

C/EBP Beta / CEBPB Antibody (clone 1D5-E5-G8, Preservative Free)

LS-C743660
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCEBPB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    C/EBP Beta / CEBPB Antibody (clone 1D5-E5-G8, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:6400000), WB (1:1000 - 1:5000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D5-E5-G8
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1051
  • Target name
    CEBPB
  • Target description
    CCAAT enhancer binding protein beta
  • Target synonyms
    C/EBP-beta, IL6DBP, NF-IL6, TCF5, CCAAT/enhancer-binding protein beta, CCAAT/enhancer binding protein (C/EBP), beta, interleukin 6-dependent DNA-binding protein, nuclear factor NF-IL6, nuclear factor of interleukin 6, transcription factor 5, transcription factor C/EBP beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEBP Beta using anti-CEBP Beta antibody (PB9171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP Beta antigen affinity purified polyclonal antibody (Catalog # PB9171) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBP Beta at approximately 42, 46 kDa. The expected band size for CEBP Beta is at 36 kDa.
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