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CENPH antibody

70R-5516
Biosynth
Product group Antibodies
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More than 130 suppliers
ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    CENPH antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CENPH using anti-CENPH antibody (A06302-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CENPH antigen affinity purified polyclonal antibody (Catalog # A06302-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CENPH at approximately 28 kDa. The expected band size for CENPH is at 28 kDa.
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Anti-CENPH Antibody Picoband(r)A06302-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetCENPH
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Western blot All lanes: CENPH antibody at 1.61 ug/ml + Mouse lung tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
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Cusabio
CENPH AntibodyCSB-PA005211ESR1HU
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LifeSpan BioSciences
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TargetCENPH
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Product group Antibodies
RayBiotech
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ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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Anti-CENPH AntibodyA43174
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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CENPH Polyclonal Antibody, Unconjugated (bs-6519R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Product group Antibodies
Bioss Antibodies
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ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCENPH
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Immunohistochemical staining of human small intestine shows weak to moderate nuclear positivity in a subset of glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-CENPH AntibodyHPA036494
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Product group Antibodies
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Centromere Protein H (CENPH) Polyclonal AntibodyCAU28234
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WB analysis of human liver tissue using GTX44860 CENPH antibody at 0.2-1microg/ml.
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CENPH antibody, InternalGTX44860
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