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WB analysis of human liver tissue using GTX44860 CENPH antibody at 0.2-1microg/ml.
WB analysis of human liver tissue using GTX44860 CENPH antibody at 0.2-1microg/ml.
WB analysis of human liver tissue using GTX44860 CENPH antibody at 0.2-1microg/ml.

CENPH antibody, Internal

GTX44860
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCENPH
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Overview

  • Supplier
    GeneTex
  • Product Name
    CENPH antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64946
  • Target name
    CENPH
  • Target description
    centromere protein H
  • Target synonyms
    CENP-H; centromere protein H; interphase centromere complex protein 35; kinetochore protein CENP-H
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3R5
  • Protein Name
    Centromere protein H
  • Scientific Description
    Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CENPH using anti-CENPH antibody (A06302-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CENPH antigen affinity purified polyclonal antibody (Catalog # A06302-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CENPH at approximately 28 kDa. The expected band size for CENPH is at 28 kDa.
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