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Western Blot analysis of HuvEc cells using CEP55 Polyclonal Antibody
Western Blot analysis of HuvEc cells using CEP55 Polyclonal Antibody
Western Blot analysis of HuvEc cells using CEP55 Polyclonal Antibody

CEP55 Antibody

CSB-PA001584
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCEP55
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Overview

  • Supplier
    Cusabio
  • Product Name
    CEP55 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55165
  • Target name
    CEP55
  • Target description
    centrosomal protein 55
  • Target synonyms
    C10orf3, CT111, MARCH, URCC6, centrosomal protein of 55 kDa, cancer/testis antigen 111, centrosomal protein 55kDa, up-regulated in colon cancer 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53EZ4
  • Protein Name
    Centrosomal protein of 55 kDa
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CEP55 using anti-CEP55 antibody (A03954-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP55 antigen affinity purified polyclonal antibody (A03954-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP55 at approximately 54 kDa. The expected band size for CEP55 is at 54 kDa.
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