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ProductsBack/CEP55 antibody [N2C3]

CEP55 antibody [N2C3]

GTX112190
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCEP55
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Overview

  • Supplier
    GeneTex
  • Product Name
    CEP55 antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.85 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55165
  • Target name
    CEP55
  • Target description
    centrosomal protein 55
  • Target synonyms
    C10orf3; cancer/testis antigen 111; centrosomal protein 55kDa; centrosomal protein of 55 kDa; CT111; MARCH; up-regulated in colon cancer 6; URCC6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53EZ4
  • Protein Name
    Centrosomal protein of 55 kDa
  • Scientific Description
    Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • An ATM-Chk2-INCENP pathway activates the abscission checkpoint. Petsalaki E et al., 2021 Feb 1, J Cell Biol
    Read more
  • Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity. Kuo TC et al., 2011 Sep 11, Nat Cell Biol
    Read more

Datasheet

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Figure 1. Western blot analysis of CEP55 using anti-CEP55 antibody (A03954-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP55 antigen affinity purified polyclonal antibody (A03954-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP55 at approximately 54 kDa. The expected band size for CEP55 is at 54 kDa.
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