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Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: CSB-PA065973(CEP55 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: CSB-PA065973(CEP55 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: CSB-PA065973(CEP55 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes

CEP55 Antibody

CSB-PA065973
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetCEP55
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Overview

  • Supplier
    Cusabio
  • Product Name
    CEP55 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55165
  • Target name
    CEP55
  • Target description
    centrosomal protein 55
  • Target synonyms
    C10orf3, CT111, MARCH, URCC6, centrosomal protein of 55 kDa, cancer/testis antigen 111, centrosomal protein 55kDa, up-regulated in colon cancer 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53EZ4
  • Protein Name
    Centrosomal protein of 55 kDa
  • Scientific Description
    Centrosomal protein of 55 kDa is a protein that in humans is encoded by the CEP55 gene. CEP55 is a mitotic phosphoprotein that plays a key role in cytokinesis, the final stage of cell division. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry; PDCD6IP competes with TSG101 for the same binding site.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CEP55 using anti-CEP55 antibody (A03954-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP55 antigen affinity purified polyclonal antibody (A03954-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP55 at approximately 54 kDa. The expected band size for CEP55 is at 54 kDa.
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