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Western blot All lanes: CEP63 antibody at 12microg/ml Lane 1: K562 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 82, 64, 58, 56 kDa Observed band size: 64 kDa
Western blot All lanes: CEP63 antibody at 12microg/ml Lane 1: K562 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 82, 64, 58, 56 kDa Observed band size: 64 kDa
Western blot All lanes: CEP63 antibody at 12microg/ml Lane 1: K562 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 82, 64, 58, 56 kDa Observed band size: 64 kDa

CEP63 Antibody

CSB-PA839376LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCEP63
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Overview

  • Supplier
    Cusabio
  • Product Name
    CEP63 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80254
  • Target name
    CEP63
  • Target description
    centrosomal protein 63
  • Target synonyms
    centrosomal protein 63kDa; centrosomal protein of 63 kDa; centrosome protein CEP63; SCKL6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96MT8
  • Protein Name
    Centrosomal protein of 63 kDa
  • Scientific Description
    Required for normal spindle assembly. Plays a key role in mother-centriole-dependent centriole duplication, through centrosomal recruitment of CEP152. Also recruits CDK1 to centrosomes. Plays a role in DNA damage response. Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and delay in mitotic progression.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CEP63 using anti-CEP63 antibody (A01661-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP63 antigen affinity purified polyclonal antibody (Catalog # A01661-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP63 at approximately 70 kDa. The expected band size for CEP63 is at 81 kDa.
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