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Western Blot Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue All lanes: CES1 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63 kDa Observed band size: 63 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue All lanes: CES1 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63 kDa Observed band size: 63 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue All lanes: CES1 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63 kDa Observed band size: 63 kDa

CES1 Antibody

CSB-PA005258LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCES1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CES1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1066
  • Target name
    CES1
  • Target description
    carboxylesterase 1
  • Target synonyms
    ACAT, CE-1, CEH, CES2, HMSE, HMSE1, PCE-1, REH, SES1, TGH, hCE-1, liver carboxylesterase 1, acyl coenzyme A:cholesterol acyltransferase, brain carboxylesterase hBr1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), carboxylesterase 2 (liver), cholesteryl ester hydrolase, cocaine carboxylesterase, egasyn, human monocyte/macrophage serine esterase 1, methylumbelliferyl-acetate deacetylase 1, monocyte/macrophage serine esterase, retinyl ester hydrolase, serine esterase 1, triacylglycerol hydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23141
  • Protein Name
    Liver carboxylesterase 1
  • Scientific Description
    Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CES1 using anti-CES1 antibody (A01741-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CES1 antigen affinity purified polyclonal antibody (Catalog # A01741-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CES1 at approximately 62 kDa. The expected band size for CES1 is at 62 kDa.
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