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Liver Carboxylesterase 1 Antibody (YA2328, Rabbit)

HY-P82583
MedChem Express
TargetCES1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Liver Carboxylesterase 1 Antibody (YA2328, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1066
  • Target name
    CES1
  • Target description
    carboxylesterase 1
  • Target synonyms
    ACAT; acyl coenzyme A:cholesterol acyltransferase; brain carboxylesterase hBr1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); carboxylesterase 2 (liver); CE-1; CEH; CES2; cholesteryl ester hydrolase; cocaine carboxylesterase; egasyn; hCE-1; HMSE; HMSE1; human monocyte/macrophage serine esterase 1; liver carboxylesterase 1; methylumbelliferyl-acetate deacetylase 1; monocyte/macrophage serine esterase; PCE-1; REH; retinyl ester hydrolase; serine esterase 1; SES1; TGH; triacylglycerol hydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Liver Carboxylesterase 1 Antibody (YA2328) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2328), targeting Liver Carboxylesterase 1, with a predicted molecular weight of 63 kDa (observed band size: 55 kDa). Liver Carboxylesterase 1 Antibody (YA2328) can be used for WB, IP, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CES1 using anti-CES1 antibody (A01741-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CES1 antigen affinity purified polyclonal antibody (Catalog # A01741-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CES1 at approximately 62 kDa. The expected band size for CES1 is at 62 kDa.
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