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Western Blot analysis of Jurkat cells using Cofilin Polyclonal Antibody
Western Blot analysis of Jurkat cells using Cofilin Polyclonal Antibody
Western Blot analysis of Jurkat cells using Cofilin Polyclonal Antibody

CFL1 Antibody

CSB-PA001739
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCFL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CFL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1072
  • Target name
    CFL1
  • Target description
    cofilin 1
  • Target synonyms
    CFL, HEL-S-15, cofilin, cofilin-1, 18 kDa phosphoprotein, cofilin 1 (non-muscle), epididymis secretory protein Li 15, p18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23528
  • Protein Name
    Cofilin-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cofilin using anti-Cofilin antibody (PB9033). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin antigen affinity purified polyclonal antibody (Catalog # PB9033) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cofilin at approximately 19 kDa. The expected band size for Cofilin is at 19 kDa.
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