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Cofilin 1 antibody

GTX102156
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCFL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cofilin 1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1072
  • Target name
    CFL1
  • Target description
    cofilin 1
  • Target synonyms
    18 kDa phosphoprotein; CFL; cofilin; cofilin 1 (non-muscle); cofilin-1; epididymis secretory protein Li 15; HEL-S-15; p18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23528
  • Protein Name
    Cofilin-1
  • Scientific Description
    Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Parkin Coordinates Platelet Stress Response in Diabetes Mellitus: A Big Role in a Small Cell. Lee SH et al., 2020 Aug 15, Int J Mol Sci
    Read more
  • Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells. Chen JF et al., 2020 Aug 5, J Pharm Biomed Anal
    Read more
  • Exosomal 2,3-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain. Chen SY et al., 2020 Apr, Stem Cells Transl Med
    Read more
  • The cofilin phosphatase slingshot homolog 1 restrains angiotensin II-induced vascular hypertrophy and fibrosis in vivo. Williams HC et al., 2019 Mar, Lab Invest
    Read more
  • Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells. Yu HI et al., 2018 Oct 25, J Pharm Biomed Anal
    Read more
  • Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells. Chou HC et al., 2018 Aug 15, Life Sci
    Read more
  • Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma. Chang SJ et al., 2018 Jun 1, Arch Biochem Biophys
    Read more
  • Identification of CRKII, CFL1, CNTN1, NME2, and TKT as Novel and Frequent T-Cell Targets in Human IDH-Mutant Glioma. Dettling S et al., 2018 Jun 15, Clin Cancer Res
    Read more
  • Merkel Cell Polyomavirus Small T Antigen Drives Cell Motility via Rho-GTPase-Induced Filopodium Formation. Stakaityte G et al., 2018 Jan 15, J Virol
    Read more
  • The putative involvement of actin-binding proteins and cytoskeleton proteins in pathological mechanisms of ketamine cystitis-Revealed by a prospective pilot study using proteomic approaches. Yang HH et al., 2017 Mar, Proteomics Clin Appl
    Read more

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Figure 1. Western blot analysis of Cofilin using anti-Cofilin antibody (PB9033). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin antigen affinity purified polyclonal antibody (Catalog # PB9033) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cofilin at approximately 19 kDa. The expected band size for Cofilin is at 19 kDa.
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