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WB analysis of recombinant CFP using GTX04275 CFP antibody. Lane 1 : 100 ng Lane 2 : 50 ng
WB analysis of recombinant CFP using GTX04275 CFP antibody. Lane 1 : 100 ng Lane 2 : 50 ng
WB analysis of recombinant CFP using GTX04275 CFP antibody. Lane 1 : 100 ng Lane 2 : 50 ng

CFP antibody

GTX04275
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
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Overview

  • Supplier
    GeneTex
  • Product Name
    CFP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5-4 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CFP using anti-CFP antibody (A00852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-937 whole cell lysate, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFP antigen affinity purified polyclonal antibody (Catalog # A00852-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CFP at approximately 51KD. The expected band size for CFP is at 51KD.
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