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WB analysis of various samples using GTX66800 CHD4 antibody. Lane1 : Hela whole cell lysate(40microg) Lane2 : NIH-3T3 whole cell lysate(40microg) Lane3 : H9C2 whole cell lysate(40microg) Lane4 : PC12 whole cell lysate(40microg) Dilution : 1:500
WB analysis of various samples using GTX66800 CHD4 antibody. Lane1 : Hela whole cell lysate(40microg) Lane2 : NIH-3T3 whole cell lysate(40microg) Lane3 : H9C2 whole cell lysate(40microg) Lane4 : PC12 whole cell lysate(40microg) Dilution : 1:500
WB analysis of various samples using GTX66800 CHD4 antibody. Lane1 : Hela whole cell lysate(40microg) Lane2 : NIH-3T3 whole cell lysate(40microg) Lane3 : H9C2 whole cell lysate(40microg) Lane4 : PC12 whole cell lysate(40microg) Dilution : 1:500

CHD4 antibody

GTX66800
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCHD4
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Overview

  • Supplier
    GeneTex
  • Product Name
    CHD4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1108
  • Target name
    CHD4
  • Target description
    chromodomain helicase DNA binding protein 4
  • Target synonyms
    ATP-dependent helicase CHD4; CHD-4; chromodomain-helicase-DNA-binding protein 4; Mi-2 autoantigen 218 kDa protein; Mi-2b; Mi2-BETA; SIHIWES
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14839
  • Protein Name
    Chromodomain-helicase-DNA-binding protein 4
  • Scientific Description
    The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHD4 using anti-CHD4 antibody (A01125-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD4 antigen affinity purified polyclonal antibody (Catalog # A01125-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD4 at approximately 260 kDa. The expected band size for CHD4 is at 218 kDa.
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