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CHIA / Amcase Antibody

LS-C332555
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCHIA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CHIA / Amcase Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 33kDa/40kDa/52kDa, while the observed MW by Western blot was 52kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB The predicted MW is 33kDa/40kDa/52kDa, while the observed MW by Western blot was 52kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27159
  • Target name
    CHIA
  • Target description
    chitinase acidic
  • Target synonyms
    acidic mammalian chitinase; AMCASE; CHIT2; lung-specific protein TSA1902; TSA1902
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMCase/CHIA using anti-AMCase/CHIA antibody (A00437-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMCase/CHIA antigen affinity purified polyclonal antibody (Catalog # A00437-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMCase/CHIA at approximately 55 kDa. The expected band size for AMCase/CHIA is at 55 kDa.
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